Photobleaching refers to the irreversible destruction of a molecule's ability to fluoresce, usually due to the absorption of light. This process is common in fluorescent dyes and biological markers, where prolonged exposure to light sources such as lasers can lead to fading of the signal, complicating the analysis of samples. In toxicology, photobleaching can impact the accuracy of experiments that rely on fluorescence to quantify the presence of toxicants.