One key question is: why are 3D cultures important in toxicology? The answer lies in their ability to mimic the cellular microenvironment more closely than 2D cultures. This results in more physiologically relevant data, particularly in terms of cellular responses, metabolism, and drug resistance. For instance, cells in a 3D environment exhibit different gene expression profiles and more accurate cell-cell and cell-matrix interactions, providing insights that are closer to in vivo conditions.