Culture methods are crucial in toxicology for several reasons. Firstly, they allow for the controlled and reproducible study of cellular responses to toxicants. Secondly, they reduce the need for animal testing, adhering to the principles of the 3Rs (Replacement, Reduction, and Refinement). Lastly, they help in understanding the molecular mechanisms of toxicity, which can lead to the development of targeted therapies and improved risk assessment models.
