Primary Cell Cultures Primary cell cultures involve the isolation of cells directly from tissues of an organism. These cells maintain many of the characteristics of the original tissue, making them highly relevant for toxicological studies. However, they have a limited lifespan and can be challenging to maintain.
Continuous Cell Lines Continuous cell lines are immortalized cells that can be cultured indefinitely. They are easier to maintain and are widely used in toxicology studies. However, they might not accurately represent the in vivo environment due to genetic and phenotypic changes over time.
3D Cell Cultures 3D cell cultures are more physiologically relevant as they mimic the three-dimensional architecture of tissues. They provide a more accurate representation of how cells interact with their environment and respond to toxicants. These cultures are gaining popularity for their improved predictive power in toxicity testing.
Organotypic Cultures Organotypic cultures involve the cultivation of tissue slices that maintain the architecture and function of the original organ. These cultures are particularly valuable for studying organ-specific toxicity and complex cellular interactions.
