Compliance with the AWA is crucial for several reasons. Firstly, it ensures the humane treatment of animals, which is an ethical obligation. Secondly, it enhances the validity and reliability of research data. Stress and pain can affect an animal’s physiological and biochemical responses, potentially skewing the results of toxicological studies. Lastly, compliance is legally mandatory; failure to adhere to AWA standards can result in penalties, including fines and revocation of research licenses.
