Spectral unmixing is essential in toxicology for several reasons. First, it enhances the accuracy of toxin detection in complex samples, where multiple compounds may interfere with each other. Second, it enables the detection of low-abundance toxins that might otherwise go unnoticed due to the presence of more dominant substances. Finally, it supports the development of new analytical methods and improves the reliability of toxicological analysis, ultimately contributing to better public health and safety.