The use of in vitro methods has become increasingly popular due to ethical considerations, cost-effectiveness, and the need for high-throughput screening. These methods allow researchers to minimize the use of animals in research, adhere to 3Rs principles (Replacement, Reduction, and Refinement), and provide rapid insights into the potential toxicity of a substance. Additionally, in vitro methods can be tailored to focus on specific cellular mechanisms, offering detailed mechanistic insights that are sometimes challenging to obtain in vivo.
