Acute toxicity tests are typically conducted using animal models such as rodents or fish. The substance of interest is administered via an appropriate route (oral, dermal, inhalation) and the animals are observed for a set period, usually 14 days, for any signs of toxicity. Parameters such as body weight, behavioral changes, and mortality are recorded. The results help in classifying the substance according to its toxicity level, which is essential for labeling and safety guidelines.
