Chronic toxicity tests are more complex and time-consuming than acute tests. They often involve exposing animals to the substance for a significant portion of their lifespan, which could be up to two years for rodents. Multiple doses are administered to establish a dose-response relationship. Parameters monitored include body weight, food and water consumption, clinical symptoms, and histopathological examination of tissues. These studies provide insights into the potential long-term effects such as carcinogenicity, reproductive toxicity, and neurotoxicity.
