The process of conducting a bioassay typically involves several key steps:
Selection of Test Organism or System: The choice depends on the goals of the study, the nature of the substance, and ethical considerations. Exposure to the Substance: Organisms or cells are exposed to varying concentrations of the substance to observe dose-response relationships. Observation and Measurement: Effects are monitored, and data are collected on endpoints such as mortality, growth, reproduction, or biochemical changes. Data Analysis: The results are analyzed statistically to determine the toxic dose and potential risks.
