The detection and quantification of DNA adducts are essential for understanding their role in disease. Techniques such as 32P-postlabeling, mass spectrometry, and high-performance liquid chromatography (HPLC) are commonly used. These methods allow researchers to identify and measure the extent of DNA adduct formation in biological samples. Advances in these techniques have improved sensitivity and specificity, making it possible to detect even low levels of DNA adducts.