The methodology of kinetic assays varies depending on the specific toxin and biological system under study. Generally, these assays involve:
Selection of Biological System: This could be an in vitro system, such as isolated enzymes or cultured cells, or an in vivo model, such as animal studies. Exposure to Toxin: The biological system is exposed to varying concentrations of the toxin, and the response is measured over time. Measurement of Response: Responses can be measured using a variety of techniques, such as spectrophotometry, fluorescence, or luminescence, depending on the nature of the reaction. Data Analysis: The collected data is analyzed to determine reaction rates, calculate kinetic parameters, and model the time-dependent effects of the toxin.
