Despite their advantages, kinetic assays also present several challenges:
Complexity of Biological Systems: The inherent complexity and variability of biological systems can complicate the interpretation of kinetic data. Technical Limitations: Accurate and precise measurement of dynamic changes over time requires sophisticated instrumentation and expertise. Data Interpretation: The large volume of data generated by kinetic assays can be challenging to analyze and interpret, especially in systems with multiple interacting components. Reproducibility: Ensuring consistent and reproducible results across different experiments and laboratories can be difficult.