Several methodologies are employed to measure T cell responses in toxicological studies:
- Flow Cytometry: This technique is used to analyze the expression of surface markers and intracellular cytokines, allowing for the assessment of T cell subsets and their activation states. - ELISA (Enzyme-Linked Immunosorbent Assay): Utilized to measure cytokine levels in the blood or culture media, providing insights into the functional status of T cells. - Proliferation Assays: These assays evaluate the ability of T cells to proliferate in response to specific antigens or mitogens, indicating their health and functionality.
