Hydrolases function by adding water molecules to substrates, leading to the cleavage of chemical bonds. This process is crucial for the breakdown of esters, amides, and glycosides, among others. The action of hydrolases can either activate or deactivate a substance, influencing its toxicity and biological activity. This enzymatic activity is significant in the metabolism of various toxins and drugs, converting them into more water-soluble compounds that can be easily excreted from the body.