In vitro methods, which involve studying biological properties outside their normal biological context, are increasingly used in toxicology. These methods rely on cell cultures or biochemical assays, allowing scientists to study cellular responses to toxic substances without using live animals. High-throughput screening (HTS) is a prime example, enabling researchers to quickly test thousands of chemicals for potential toxicity. These techniques provide valuable data on cellular and molecular mechanisms of toxicity and are crucial for initial toxicity screening.