The BCOP assay involves the use of corneas extracted from bovine eyes obtained from slaughterhouses. The corneas are mounted in specially designed holders, and the test substance is applied to the corneal surface. The assay measures two primary endpoints: corneal opacity and corneal permeability. Opacity is assessed by measuring the amount of light passing through the cornea, while permeability is determined by evaluating the uptake of a dye, such as fluorescein, indicating damage to the corneal epithelial barrier.
