The process of determining LOD involves several steps, typically including:
1. Calibration Curve: Preparing a calibration curve using known concentrations of the analyte. 2. Signal-to-Noise Ratio: Establishing a signal-to-noise ratio (S/N) where the LOD is often defined at a S/N of 3:1. 3. Replicate Measurements: Analyzing replicate measurements of samples with low concentrations of the analyte to assess variability.
These steps help in generating reliable and reproducible LOD values, ensuring the credibility of the analytical method.
