Phosphoproteomics involves several key steps: sample preparation, enrichment of phosphorylated proteins, mass spectrometry analysis, and data interpretation. First, proteins are extracted from biological samples, and phosphorylated proteins or peptides are enriched using techniques such as IMAC or MOAC. These enriched samples are then analyzed using mass spectrometry, which identifies and quantifies the phosphorylated peptides. Finally, bioinformatics tools are used to interpret the data and map the phosphorylation sites to specific signaling pathways.
