What are the Challenges Associated with Fluorescent Labeling?
Despite its advantages, fluorescent labeling poses certain challenges. The primary concern is photobleaching, where the fluorescent dye loses its ability to emit light over time. This can limit the duration of observation and affect quantitative measurements. Another issue is the potential for non-specific binding of the dye to unintended targets, which can lead to misleading results. Additionally, the introduction of fluorescent labels can sometimes alter the biological activity of the target molecules.
