What are the Limitations of Enzyme Histochemistry in Toxicology?
Despite its advantages, enzyme histochemistry has limitations. It often requires fresh or well-preserved tissue samples, as enzyme activity can diminish over time. The technique is also limited by the specificity of substrates used; not all enzymes have specific substrates available for histochemical detection. Furthermore, the subjective nature of interpreting results can introduce variability, necessitating the need for experienced personnel to ensure accurate data analysis.
