fluorescence recovery after photobleaching (frap)

What Are the Limitations of FRAP in Toxicology?

Despite its advantages, FRAP has certain limitations. It requires the use of fluorescent tags, which might alter the native behavior of the molecules being studied. The technique also requires specialized equipment and expertise to accurately interpret the results. Additionally, FRAP is predominantly a qualitative tool, and complementary quantitative methods may be necessary to fully understand the molecular changes induced by toxicants.

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