What are the Limitations of PAS Staining in Toxicology?
While PAS staining is a powerful tool, it does have limitations. It cannot provide specific information about the chemical nature of the substances being stained, requiring complementary techniques for a more comprehensive analysis. Additionally, PAS staining may not differentiate between similar polysaccharides and might produce false positives if not carefully controlled. Therefore, it is often used in conjunction with other histochemical and biochemical assays to validate findings.