Periodic Acid Schiff (PAS) staining is a histochemical technique used to detect polysaccharides such as glycogen, mucosubstances such as glycoproteins, glycolipids, and mucins in tissues. It involves the oxidation of the glucose ring to generate aldehydes, which then react with the Schiff reagent to produce a distinct purple-magenta color. This staining method is widely used in various fields, including toxicology, to assess tissue changes due to exposure to toxic substances.
