What are the Limitations of the Shake Flask Method?
Despite its advantages, the shake flask method has some limitations:
Time-Consuming: The method can be labor-intensive and time-consuming, particularly for compounds that achieve equilibrium slowly. Accuracy Issues: It may not be suitable for volatile or very hydrophilic compounds due to potential losses or inaccuracies in concentration measurements. Solvent Limitations: The choice of organic solvent can influence results, and not all solvents are compatible with this method.
