What are the Limitations of Using γ H2AX as a Biomarker?
While γ H2AX is a sensitive indicator of DNA damage, it does have limitations. For example, γ H2AX formation can also occur during replication stress and apoptosis, not just due to DSBs. Additionally, the persistence of γ H2AX foci can vary depending on the efficiency of the DNA repair mechanisms. Therefore, complementary assays may be needed to confirm the nature and extent of the DNA damage.
