What are the Steps Involved in Performing a Comet Assay?
Performing a comet assay involves several steps:
Cell Preparation: Isolate cells from the tissue or culture. Embedding in Agarose: Embed the cells in low-melting-point agarose on a microscope slide. Lysis: Treat the slides with a lysis solution to remove cell membranes and proteins. Electrophoresis: Subject the nucleoid to an electric field to allow DNA migration. Staining: Stain the DNA with a fluorescent dye such as ethidium bromide. Analysis: Examine the slides under a fluorescence microscope and analyze the comet tail length and intensity.
