The BCOP assay involves the use of isolated bovine corneas obtained as by-products from abattoirs. The corneas are mounted in a specialized chamber, and test substances are applied directly to the corneal surface. The test measures two primary endpoints: corneal opacity and corneal permeability. Opacity is measured as the reduction in light transmission through the cornea, while permeability is assessed by the uptake of a dye such as fluorescein, which penetrates damaged corneal layers.
