The BCOP assay follows a standardized procedure. Fresh bovine eyes are sourced from abattoirs, and corneas are carefully dissected and mounted in a corneal holder. The test substance is then applied to the corneal surface for a specified exposure period. After exposure, the corneas are rinsed, and opacity is measured using a spectrophotometer. Permeability is assessed by applying fluorescein and measuring the optical density of the dye that has penetrated the cornea. The results are then interpreted to classify the irritancy potential of the test substance.
