The future of ITS in toxicology is promising, with continuous advancements in technology and science. The integration of new techniques, such as omics technologies and artificial intelligence, is expected to enhance the predictive capabilities of ITS. Furthermore, ongoing efforts to refine and validate existing models will increase their acceptance and use in regulatory contexts. As the demand for safer chemicals grows, ITS will play a pivotal role in ensuring public and environmental health while minimizing the reliance on animal testing.