The process of glutathione conjugation involves several steps:
1. Recognition: The enzyme glutathione S-transferase (GST) identifies and binds to the electrophilic toxicant. 2. Conjugation: GST catalyzes the conjugation of GSH to the toxicant, forming a glutathione-toxin conjugate. 3. Transport: The conjugate is transported out of the cell by ATP-dependent transporters. 4. Excretion: Finally, the conjugate is excreted from the body, usually through urine or bile.
