The construction of a PBPK model involves several key steps: 1. Compartmentalization: The body is divided into different compartments representing various tissues and organs. 2. Parameterization: Physiological parameters such as blood flow rates, tissue volumes, and partition coefficients are determined. 3. Mathematical Equations: Differential equations are formulated to describe the movement of the chemical between compartments. 4. Validation: The model is validated using experimental data from in vitro and in vivo studies.
