There are several methods to detect lipid peroxidation, each with its advantages and limitations. One common method is the thiobarbituric acid reactive substances (TBARS) assay, which measures MDA levels. Another method involves high-performance liquid chromatography (HPLC) to quantify specific lipid peroxidation products like HNE. More advanced techniques include mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, which can provide detailed information about the specific molecules involved.
