Diagnosis of optic nerve toxicity involves a combination of clinical evaluation, patient history, and specialized tests. A thorough ophthalmologic examination is essential to assess visual acuity, color vision, and visual fields. Imaging techniques like optical coherence tomography (OCT) and magnetic resonance imaging (MRI) can provide detailed views of the optic nerve structure and any associated damage. Blood tests may be conducted to identify the presence of toxins or metabolic disturbances.