Detection and quantification of pneumolysin can be performed using several techniques. Enzyme-linked immunosorbent assay (ELISA) is commonly used to measure pneumolysin levels in biological samples. Other methods include Western blotting, mass spectrometry, and polymerase chain reaction (PCR) to detect the presence of pneumolysin genes. These techniques are crucial for understanding the role of pneumolysin in disease and evaluating the efficacy of therapeutic interventions.
