1. Cell Culture: Cells are cultured in appropriate conditions and exposed to the test substance. 2. Incubation: After an incubation period, the cells are subjected to a lysis buffer to release intracellular LDH. 3. Reaction: The released LDH catalyzes the conversion of lactate to pyruvate, producing NADH in the process. 4. Measurement: The amount of NADH produced is quantified using a spectrophotometer, providing an indirect measure of LDH activity and, consequently, cell damage.
