When determining the best method, toxicologists consider several factors:
Objective of the Study: Is the study focused on acute toxicity, chronic toxicity, or a different endpoint? The objective will guide the selection of the method. Nature of the Substance: Chemical properties of the substance, such as solubility and volatility, can influence the choice of method. Ethical Considerations: With increasing emphasis on reducing animal testing, alternatives like in vitro or in silico methods are preferred whenever possible. Regulatory Requirements: Different regions may have specific guidelines on the type of testing required for certain substances.
