1. Sample Preparation: Cells are cultured and treated with the test substance. 2. Incubation: Cells are incubated to allow for potential LDH release. 3. Lysis: Cells are lysed to release intracellular LDH. 4. Reaction: The culture medium is mixed with LDH assay reagents. 5. Detection: The reaction mixture is incubated, and the absorbance or fluorescence is measured.
