What are the Advantages of Using In Situ Hybridization in Toxicology?
ISH offers several advantages in toxicological research:
High specificity and sensitivity: ISH provides precise localization of target sequences, allowing for the detection of specific mRNA transcripts within a complex tissue environment.
Spatial information: Unlike other molecular techniques, ISH preserves the spatial context of gene expression, which is crucial for understanding the pathophysiology of toxicant-induced damage.
Compatibility with archival samples: ISH can be performed on fixed, paraffin-embedded tissue samples, making it possible to study historical specimens and conduct retrospective analyses.
