Are There Any Limitations to In Situ Hybridization?
While ISH is a valuable tool in toxicology, it does have some limitations:
Technical complexity: ISH protocols can be complex and require careful optimization of various parameters such as probe design, hybridization conditions, and detection methods.
Quantification challenges: Although ISH can provide qualitative information on gene expression, quantifying expression levels can be challenging and often requires additional techniques such as image analysis software.
Time-consuming: The ISH process can be time-consuming, particularly when optimizing conditions for new probes or targets.
