The study of hydrolases in toxicology is complex due to several factors:
There are numerous hydrolase isoforms with overlapping substrate specificities, making it challenging to pinpoint specific enzyme-substrate interactions. Environmental factors, such as diet and exposure to other chemicals, can modulate hydrolase activity, complicating the interpretation of toxicological data. In vitro studies may not always accurately reflect in vivo conditions, leading to challenges in extrapolating experimental findings to real-world scenarios.
