What are the Limitations of Fluorescence Spectroscopy?
While highly advantageous, fluorescence spectroscopy also has limitations:
Quenching: The fluorescence signal can be quenched by various factors, including the presence of other chemicals or changes in the environment such as pH and temperature. Background Signals: Biological samples often contain naturally fluorescent compounds, which can interfere with the detection of the target analyte. Complex Sample Preparation: In some cases, extensive sample preparation may be required to isolate the target substance and reduce interference.
