Detecting and analyzing metabolites often involve advanced techniques such as:
Mass Spectrometry (MS): A powerful tool for identifying and quantifying metabolites based on their mass-to-charge ratio. It is often coupled with chromatographic techniques for separation. Liquid Chromatography (LC): Used to separate complex mixtures of metabolites before detection by MS or other detectors. Nuclear Magnetic Resonance (NMR) Spectroscopy: Provides structural information about metabolites, which can be crucial for identifying unknown substances.