In toxicology, live cell imaging offers several advantages. It provides a window into the mechanism of action of toxic agents, allowing researchers to pinpoint how these substances affect cellular structure and function. This information is crucial for understanding dose-response relationships and potential adverse effects. Additionally, live cell imaging can be used to monitor the real-time effects of toxins on cell viability, apoptosis, and necrosis, offering a more comprehensive view of cellular responses than endpoint assays.