Subacute toxicity testing typically involves the use of animal models to evaluate the effects of a substance. Here are the main steps involved:
Selection of Species: Commonly used species include rodents, such as rats and mice, due to their well-characterized physiology and ease of handling. Administration Route: The chemical is administered through relevant routes of exposure (e.g., oral, dermal, inhalation) that mimic potential human exposure scenarios. Dosage Levels: Different dosage levels are chosen to observe a range of effects, including a control group receiving no exposure. Observation Period: Animals are observed for clinical signs of toxicity, changes in body weight, organ weights, and biochemical parameters over the study period. Data Analysis: Collected data is analyzed to determine the NOAEL (No Observed Adverse Effect Level) and LOAEL (Lowest Observed Adverse Effect Level), which are critical for risk assessment.
