While AR assays are invaluable tools, they do have limitations. One major limitation is that they typically do not account for metabolic activation or degradation of the test compound, which can influence its activity in vivo. Additionally, AR assays often focus on a single receptor subtype, whereas complex in vivo systems may involve multiple receptor interactions and feedback mechanisms. Lastly, these assays generally do not provide information on the long-term effects of exposure to the compound.
