Despite its importance, studying the apoptosome poses several challenges. Its complex structure and transient nature make it difficult to study using traditional biochemical techniques. Additionally, the apoptosome's activity can be influenced by a myriad of factors, including genetic mutations, post-translational modifications, and interactions with other cellular proteins. These complexities necessitate advanced techniques, such as cryo-electron microscopy, to elucidate its structure and function in detail.
